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Judul: Perancangan Primer-oligonukleotida Untuk Reaksi Rantai Polimerisasi Gen Sukrosa Sintase (EC 2.4.1.13)
Penulis: Pieter Agusthinus Riupassa  || email: info@mx.unpatti.id
Jurnal: Prosiding FMIPA Universitas Pattimura 2010 Vol. 1 no. 1 - hal. 21-29 Tahun 2010  [ MIPA ]
Keywords:  Sucrose synthase, Primer, Oligonucleotide, PCR, Bioinformatics
Abstract: The most important problems in using polymerase chain reaction (PCR) are the efficiency of energy, cost and time due to amplify gene. Primer-oligonucleotide design of sucrose synthase gene was conducted as a model of preliminary experiment to amplify gene using PCR. In plant cells, this gene has a play important role in carbohydrate metabolism; it breaks down a sucrose molecule into glucose. This design were involved some computer software in bioinformatics tools. Five data sequences of legumes are downloaded from gene bank using accession number AF030231, AJ311496, X92378, X69773, and D10266 which belonging to soybean, pea, alnus bean, fava bean, and mung bean, respectively. After sequences alignment, some conservative regions were determined as forward and reverse primer candidates. Furthermore, the candidates were tested for primer oligonucleotides compatibility. The results showed that the primer oligonucleotides can amplify sucrose synthase gene with ? 1462 bp fragment size using 5'- AACTTTgTgCTTgA-3' and 5'-TCCTTTgACTCCTTC-3' for forward and reverse primer, respectively. Even though the PCR process were not applied, those primers might be a universal primer to amplify sucrose synthase gene of legume plants.
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