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Judul: Ekstraksi dan Identifikasi Polisakarida Bersulfat Dari Sayur Karang Gracilaria sp
Penulis: Dominggus Malle, Eirene Grace Fransina & Feky Jansen  || email:
Jurnal: Indonesian Journal of Chemical Research Vol. 1 no. 2 - hal. 83-87 Tahun 2014  [ MIPA ]
Keywords:  Extraction, Sulphated polysaccharide, Gracilaria, FTIR
Abstract: Polysaccharide from Gracilaria sp (locally known as “Sayur Karang”) was extracted using hot alkaline solutions (NaOH and KOH). Fractionation using DEAE-Sepharose Fast Flow column showed that the polysaccharide discharged after being eluted with 0.5 – 1.0 M NaCl. Spectroscopic analysis using FTIR showed that the polysaccharide contained hydroxyl (3420 cm-1 (NaOH frcation) dan 3446 cm-1 (KOH frcation)), carbonyl (1642 cm-1 (NaOH frcation) and 1644 cm-1 (KOH frcation)) and sulphate (1271 cm-1 (NaOH frcation) and 1262 cm-1 (KOH frcation)) groups proving that the polysaccharide is a sulphated polysaccharide. A further study is needed to elucidate the structure of the sulphated polysaccharide as well as physiochemical properties of the polysaccharide.
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