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| Judul: | The Use of Fish as Carbon Sources for The Production of Riboflavin (Vitamin B2) Using Eremothecium Gossypii |
| Penulis: | Syarifuddin Idrus, Marni Kaimudin & Joice P. M. Kolanus || email: syarif.idrus@gmail.com |
| Jurnal: | Prosiding FMIPA 2015 Vol. 1 no. 1 - hal. 41-49 Tahun 2015 [ MIPA ] |
| Keywords: | Riboflavin, vitamin B2, Eremothecium gossypii, fermentation |
| Abstract: | Riboflavin or vitamin B2 is a vitamin which used as a nutritional, therapeutic, and livestock feed supplements.In techno economy, the cost of riboflavin production by fermentation were cheaper, less waste generated, and lower the energy required compared to the chemical and fermentation-chemical processes.In addition, the fermentation process also uses substrate from renewable sources.This study aims to produce riboflavin using fish as a substrate.Fish that contain fatty acids and proteins are expected to be a source of carbon for the production of riboflavin by fermentation using Eremothecium gossypii.The fermentation process was observed at 0-120 hours to obtain an optimal time and substrate for the production of riboflavin.Riboflavin was tested using a spectrophotometer at a wavelength of 445 nm.The results showed that the optimal time for the production of riboflavin was 72 hours.Glucose as a carbon source, optimal at 10 g/L with a number of riboflavin obtained amounted to 40.5 mg/L.The use of fish as a source of carbon, optimal at 10 g/L with the amount of riboflavin was 24.8 mg/L.The use of a mixture of glucose and fish as carbon sources, optimal at 10 g/L with the amount of riboflavin was 51.2 mg/L. |
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