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Judul: Isolation and Identification of Lipase Producing Thermophilic Bacteria From A Hot Spring At Seram Island, Moluccas
Penulis: Edwin Thomas Apituley, Nisa Rachmania Mubarik & Antonius Suwanto  || email:
Jurnal: Prosiding FMIPA 2016 Vol. 1 no. 1 - hal. 213-218 Tahun 2016  [ Prosiding ]
Keywords:  Thermophilic bacteria, lipase, Geobacillus stearothermophilus.
Abstract: Lipases are enzyme that widely used in fat and oil processing, synthesis of fine chemichals and pharmaceuticals, food processing and lipid rich waste water treatment. Lipases from microbial sources prefered for biotechnological fields application due to their ability to catalyze a wide variety of hydrolitic and synthetic reaction. Thermophilic bacteria often possess enzyme that are stable and active at high temperature which are valuable for some biotechnological and industrial applications such as lipase enzyme for hydrolysis and esterification reaction. High temperature environment usally associated with volcanic phenomenas such as hot spring or geothermal vents. Isolat T 1.2 was thermophilic bacterium isolated from a hot spring at Seram island, which had temperature 89°C and pH 6.3. Isolation was conducted using Basal Salt Medium suplemented with olive oil, and Rhodamin B media. Comparison of 16SrDNA sequence to GenBank database using Basic Local Alignment Search Tools for nucleotides (BLASTn) showed that isolat T1.2 was closely related to Geobacillus stearothermophilus with 99% homology. The phylogenetic tree of 16SrRNA was constructed using Neighbour Joining Method and distance was estimated using Kimura’s two parameter method with one hundred bootstrap replicates showed that the thermophilic isolat T1.2 is clustered to other members of Geobacillus and closely related to Geobacillus stearothermophilus.
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