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| Judul: | Essential Oil Compound Extract Of Six Temu-Temuan (Curcuma spp) Plants as Antibacterial Agent |
| Penulis: | Maiyani Hartono, Nurlaila & Irmanida Batubara || email: maiyani.hartono@gmail.com |
| Jurnal: | Prosiding PERMAMA 2012 Vol. 1 no. 1 - hal. 61-74 Tahun 2012 [ Prosiding ] |
| Keywords: | Essensial oil, antibacterial, Curcuma heyneana, Curcuma aeruginosa, Boesenbergiia pandurata, Curcuma xanthorrhiza, Curcuma mangga, Curcuma zeodariam, Staphylococcus epidermis |
| Abstract: | The objective of this research was to see the components of six temu- temuan Plants (_) essential oil extract: temu giring (_)/TI, temu kunci )/TL, temu )/TP as , and _. Essential oil with high yield was separated with chromatography column for fractionation. Each active fraction was determined and analyzed by GC-MS. The result shows that each yields from TG, TI, TK, TL, TM, and TP 1.00%, 0.47%, 1.33%, 0.47%, 1.00%, and 0.47%, MIC on _ for TK and TG 1000 mg/ml, TI, TM, TP 500 mg/ml, and TL 125 mg/ml. The most active MIC is TL with 125mg/L, and MBC is TG with 1000 mg/ml concentration. TK was the highest )/TG, temu Ireng ( (_)/TK, temu lawak ( mangga (_)/TM, and temu putih ( antibacterial agents. The bacteria used were _ for essential oil yield from the six _. plants. All essential destilation extracts were tested for antibacterial activity with micro plate method. All _ essential oil destilation extracts with minimum inhibitory concentration (MIC) gave the following results: TK and TG 1000 mg/ml, TI, TM, and TP 500 mg/ml, and TL 125mg/ml. TG were the most active with MIC 1000 mg/ml and minimum bactericidal concentration (MBC) 1000 mg/ml. All volatile oil  were obtained by water distillation. Chemical components were determined using gas chromatography and mass spectrometry (GC-MS). From the positive results of MIC and MBC, the TG main components of volatile oil were obtained as follows: 1.8-Cineole, Camphene, Camphor, Borneol L, 1- - -elemene, Methoxymethyl, Xanthorhizol, Germacrene, 1- -terpineol, caryophyllene oxide, 6-methylxanthotoxin, critonilide, 2-methoxymethyl-4,4-dimethyl-5-phenyldihydro pyran. |
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