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Judul: Pemanfaatan Verticillium tricorpus Sebagai Agen Pengendalian Hayati Penggerek Buah Kakao Conopomorpha cramerella di Jayapura Provinsi Papua
Penulis: B.E.L.L. Gomies  || email:
Jurnal: Budidaya Pertanian Vol. 5 no. 2 - hal. 99-104 Tahun 2009  [ Pertanian ]
Keywords:  Exploiting, control, natural, borer, cacao
Abstract: This research is occurred in Laboratory of Plantation department of Jayapura, Papua Province of since July to September 2008. Objective of this research are to known: 1) Effective spore concentration of V. tricorpus to infect to stadia larva, pupa and imago of C. cramerella, 2) most infected stadia by V. tricorpus 3) effective application interval in field test. Culture V. tricorpus used for the multiplying is collected on the Laboratory of Biological Protection Plantation Department of Papua Province. The isolate of V. tricorpus are multiplied on rice media to get the V. tricorpus culture which is high virulence. Formula spore are count used haemacytometer. Spore concentration of larva, pre pupa, pupa and imago mortality conducted by dipping it on concentration of according to treatment that are 10-4, 10-5, 10-6, 10-7 spore ml-1 concentration and control. This research is compiled in Completely Randomized Designed (CRD) with five treatments, with three times replication. Field examination compiled in Complete Random Design consisted with five treatments and three times replication. The treatments is spraying interval, 1 times, 2 times, 3 times, 4 times (certain 7 days period) and without spraying/control. Based to result statistical analyze of larva, pre pupa, pupa and imago mortality at 8 days after treatment has not marked real different between treatment. It showed by the result test of larva of pre pupa, pupa and imago mortality of 10-4, 10-5, 10-6, 10-7 spore ml-1 concentration do not marked significant different but different with control. The lowest concentration of spore is 10-7 spore ml-1 which caused the highest mortality at stadia of larva of pre pupa, pupa and imago. This concentration used in field application. Pre pupa stadium is the high mortality at laboratory test it is shown as a result of LC50 and LC95 calculation. LC50 for the pupa stadium more lower compared with imago. At field examination the lowest concentration used for each time application. The highest percentage of fruit attacked is 1 times application of treatment. Statistical result analyze marked real different, between treatment of application interval 1, 2, 3 and 4 times applications. Result shown that fruit percentage attacked by PBK of application treatment every 7 day marked the reality different with control of but not different between treatments. Field test result shown that to get the free fruit cacao from the PBK is by application the V. tricorpus every 4 times on 7 days, because the long time application will broke life cycle of PBK pest.
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